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Protocols for Molecular Biology and Protein Chemistry

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Protein Chemistry

Bullet Tricine-SDS

Bullet Native Acrylamide Gels

Bullet TCA Precipitation

Bullet Examining soluble/insoluble partitioning of overexpressed proteins

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Preparation of 15N and 13C Labeled Protein

Bullet Minimal Salts Media

Bullet Growth of Prototrophic E. coli Strains in D2O Minimal Salts Media

Bullet Buffer Exchange Procedure for the Preparation of NMR Samples

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Molecular Biology

Bullet Quick Ligation Protocol

Bullet Preparation of Chemically Competent Cells & Transformation of a Ligation Mixture

Bullet Preparation of Electrocompetent Cells

Bullet Electroporation Procedure

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Protein Preps

Bullet ecTbR2 Expression, Folding, and Purification

Bullet TGF-b3 Expression, Folding, and Purification

Bullet N-terminal HisTagged TGF-b3 Expression, Folding, and Purification
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Last updated on February 29, 2004